Only put the controls necessary for creating that compensation matrix in this compensation matrix. One compensation group will yield one compensation matrix from the controls provided to that group. If you require multiple new compensation matrices, simply create a new group and assign it a role of ‘compensation’.
There is a single compensation matrix in the workspace by default and it will allow you to create one new compensation matrix in FlowJo. Compensation controls (single stain samples) must now belong to a compensation group in order to create a compensation matrix with these controls. Chapter 2 – Creating a New Compensation Matrix in FlowJo CHAPTER 2 – Creating a FlowJo compensation matrix from single stain controlsīefore compensating data in FlowJo, be sure your parameters which require compensation are scaled correctly.Ĭompensation has been reworked for version 10.
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